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What is reverse-sorted semen?

February 24, 2020

Frozen semen is thawed, and our semen sorter is utilized to separate the female and male sperm cells. We call this process "reverse-sorting," because the sorting occurs after the semen has been previously frozen. The sorted semen of the desired gender is then used to fertilize the oocytes collected from donors. This process generally requires a minimum of two units of semen for a given sire.

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What results can be expected from reverse-sorted semen?

February 24, 2020

The reverse sorting process can provide samples for fertilization with approximately 87-90% accuracy of the desired sperm cells. Embryos created with reverse sorted semen have been reported on average to be up to 90% accurate on the desired gender. Although most sires will sort accurately, the process can be affected by semen quality and concentration thus resulting in variation in embryo/live calf sex ratio. There is often significant variation among sires in fertility and embryo development rates.

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What are the pregnancy rates when IVF embryos are transferred fresh?

February 24, 2020

On average, we expect fresh IVF embryos to achieve about a 45-50% pregnancy rate. This will vary somewhat depending on the time of year, type of recipient, and recipient management.

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What results can be expected on each IVF cycle?

February 24, 2020

Results vary with each donor, but we typically expect to collect ~18 oocytes per aspiration. On average, 30% of these oocytes will develop into a viable embryo. Thus, we expect about 5 transferrable (Grade 1 & 2) embryos per IVF cycle on average. Donors that produce greater numbers of oocytes and oocytes of higher quality may see larger numbers of embryos produced, whereas donors with compromised reproductive conditions may have lower results. Development rate will also vary greatly depending on the sire used.

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What results can be expected with different semen types?

February 24, 2020

On a given sire, the development rate between conventional and reverse-sorted semen is generally very similar. However, we do see a decrease in development rate with pre-sexed frozen semen compared to reverse-sorted semen with many bulls. Thus, our recommendation for clients wanting to produce sexed embryos is to use reverse-sorted semen.

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